| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 481 | Acidithiobacillus ferrooxidans | AAATACGCGTCCTGCCGA | TCACTTTCGCCTGGCAACT | 59.43 | 59.85 | 267 | 65 |
100.00%
|
100.00%
|
| Primer 482 | Acidithiobacillus ferrooxidans | AGCGCTGCAACAACCTGA | AGCGAGAAACTGGGCTGT | 60.20 | 58.85 | 299 | 65 |
100.00%
|
100.00%
|
| Primer 483 | Acidithiobacillus ferrooxidans | TGGCAACGGCATGAACGA | CGCACCTCGTCCAGTTCTT | 60.28 | 60.01 | 165 | 65 |
100.00%
|
100.00%
|
| Primer 484 | Acidithiobacillus ferrooxidans | TGGCAACGGCATGAACGA | CCGGTGTGCCTTTCTCCTT | 60.28 | 59.93 | 236 | 65 |
100.00%
|
100.00%
|
| Primer 485 | Acidithiobacillus ferrooxidans | ATTTGCACCCTGGCACGA | TGTCCGGTCGTGCAAACA | 59.88 | 59.81 | 212 | 65 |
100.00%
|
100.00%
|
| Primer 486 | Acidithiobacillus ferrooxidans | TGCGTGGCAAGCATAAGC | GCACTCTTCAGGTTGCCGA | 59.12 | 60.30 | 154 | 65 |
100.00%
|
100.00%
|
| Primer 487 | Acidithiobacillus ferrooxidans | AAAATTCGCCTGCGTGCC | ACGCTGCGCTAATCCTGA | 59.74 | 59.11 | 151 | 65 |
100.00%
|
100.00%
|
| Primer 488 | Acidithiobacillus ferrooxidans | AAGTTCAGGACCGTCGCTG | AAGCGATGGCCTTGCTCA | 60.01 | 59.65 | 166 | 65 |
100.00%
|
100.00%
|
| Primer 489 | Acidithiobacillus ferrooxidans | TTCTGCGTTGCGGAGTGA | AGACGGCTTGCAGCACAT | 59.58 | 59.97 | 182 | 65 |
100.00%
|
100.00%
|
| Primer 490 | Acidithiobacillus ferrooxidans | TGCTGCTCGCTTTCCTGA | CCGAGAATGACGAAGCCGA | 59.26 | 59.86 | 175 | 65 |
100.00%
|
100.00%
|